Simultaneous quantifications of four purine derivatives biomarkers in cow milk by SPE HPLC-DAD

In this study, simultaneous quantification of allantoin, uric acid, xanthine, and hypoxanthine in cow milk by solid phase extraction (SPE) high performance liquid chromatography-diode array detection (HPLC-DAD) method was perform. Five different SPE cartridges were tested order to evaluate the isolation purine derivatives (PD) from milk. Chromatography carried out on ODS-2 Hypersil column 0.05 M (NH4)2HPO4 buffer solution (pH = 7.76) as mobile phase. The HPLC-DAD validated showed a linearity with regression coefficients higher than 0.999 limits values range 0.09–0.74 µg mL–1 0.27–2.24 mL–1, respectively. good precision relative standard deviation (RSD) below 4.48%, while accuracy ranged 95.34 104.47% for all analytes. best recovery degree PD obtained Strata SCX cartridge xanthine (87.79%) (89.02%); NH2 allantoin (35.09%) C8 acid (101.08%). Finally, applied quantify batch thirty samples.